High-resolution three-dimensional reconstruction of a whole yeast cell using focused-ion beam scanning electron microscopy

We developed an approach for focused gallium-ion beam scanning electron microscopy with energy filtered detection of backscattered electrons to create near isometric voxels for high-resolution whole cell visualization.Specifically, this method allowed us to create three-dimensional volumes of high-pressure frozen, freeze-substituted Saccharomyces c

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Identifying lipid particle sub-types in live Caenorhabditis elegans with two-photon fluorescence lifetime imaging

Fat metabolism is an important modifier of aging and longevity in Caenorhabditis elegans.Given the anatomy and hermaphroditic nature of C.elegans, a major challenge is to distinguish fats that serve the energetic needs of the parent from those that are allocated to the progeny.Broadband coherent anti-Stokes Raman scattering (BCARS) microscopy has r

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